Primer

Part:BBa_K4790042:Design

Designed by: Yi Zheng   Group: iGEM23_NNU-CHINA   (2023-08-14)


ASR1-M40E-F


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Ⅰ The last base at the 3' end of the primer is selected as C or G.

Ⅱ Tm value of the primer should be controlled between 55℃ and 65℃.

Ⅲ The GC content of primers should be controlled between 40% and 60%.

Ⅳ Forward and reverse primer Tm values and GC content should be as consistent as possible.

Ⅴ The length of primer should be no more than 42bp generally.

Ⅵ △Tm should be limited to 2℃.



Source

It does not come from any genomic sequence but is designed according to node 59(BBa_K4790064).

References